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目的:对不同粒度玉米秸秆的纤维素酶水解条件进行了探索。方法:研究了秸秆酶解产生还原糖量随时间的变化趋势,并做了粒度、温度、纤维素酶用量、固液比等因素对秸秆酶解影响的研究,通过扫描电镜(SEM)观察酶解不同粒径秸杆粉的表面形态。结果:随着玉米秸杆粉粒径的减小,其酶纤维的能力提高,即还原糖得率提高,酶解不同粒径的秸杆粉表面形貌也有一定的差异。结论:超微粉碎技术可以影响玉米秸杆中纤维的酶解效率,提高了还原糖得率,在食品及生物能源加工业应用方面具有一定的潜力。
Objective: To explore the conditions of cellulase hydrolysis of corn stalks with different particle sizes. Methods: The change trend of reducing sugar yield with straw enzymolysis over time was studied. The effect of particle size, temperature, amount of cellulase and solid-liquid ratio on the enzymatic hydrolysis of straw was studied. The enzyme Solution of different particle size straw powder surface morphology. Results: With the decrease of corn stalk particle size, the enzyme fiber capacity increased, that is, the yield of reducing sugar increased, enzymatic hydrolysis of different particle sizes of straw flour surface morphology are also some differences. Conclusion: The technology of ultrafine grinding can affect the enzymatic hydrolysis efficiency of corn stalks and increase the yield of reducing sugar, which has some potential in the application of food and bioenergy processing industry.