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雄性不育性状被用在植物育种中,以降低种子生产成本。雄性不育分为细胞核雄性不育(GMS)和细胞质雄性不育(CMS)2种类型,在辣椒商业育种中都有应用。因为缺少恢复源,细胞质雄性不育系统在诸如甜椒等一些辣椒品种上并不适用。因此,细胞核雄性不育对于甜椒的种子生产而言就非常重要。本研究构建了一个F2分离群体,利用集群分离分析法(BSA)和扩增片段长度多态性(AFLP)技术获得了一个与甜椒细胞核雄性不育连锁的标记。我们分析了1024对AFLP引物,发现Eco RI ACG/MseIGTT引物扩出了多态性。对多态性AFLP标记所扩增的395bp片段重新设计引物,进行高分辨率熔解曲线分析(HRM)。在92个F2单株中,有87个单株的性状和HRM标记表型相一致。这个HRM标记被定位在AC99连锁图的第5号染色体上。
Male sterility traits are used in plant breeding to reduce seed production costs. Male sterility is divided into two types of nuclear male sterile (GMS) and cytoplasmic male sterile (CMS), which are all used in commercial breeding of pepper. Because of the lack of recovery sources, the cytoplasmic male sterility system is not suitable for some pepper varieties such as sweet pepper. Therefore, nuclear male sterility is very important for seed production of sweet pepper. In this study, an F2 segregating population was constructed and a marker linked to the male sterility of sweet pepper nucleus was obtained by cluster analysis of segregation (BSA) and amplified fragment length polymorphism (AFLP). We analyzed 1024 pairs of AFLP primers and found that the EcoRI ACG / MseIGTT primer amplified polymorphisms. Primers were redesigned for the 395 bp fragment amplified by the AFLP marker and analyzed by high resolution melting curve analysis (HRM). Among 92 F2 individuals, 87 individuals were consistent with the HRM marker phenotype. This HRM marker is mapped on chromosome 5 of the AC99 linkage map.