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目的建立饮料中阿斯巴甜、糖精钠和安赛蜜的毛细管电泳-紫外检测法。方法样品经超声脱气,12 000r/min离心5 min后,取上清液直接进样分析。以熔融石英毛细管(50 cm×50μm,有效长度41 cm,未涂层)为分离柱;20 mmol/L硼砂溶液(pH=9.3,15%乙腈)为运行缓冲液,进样时间10 s,进样高度20 cm,分离电压15 kV,在210 nm的波长下,阿斯巴甜、糖精钠和安赛蜜在12 min内得以基线分离。结果阿斯巴甜、糖精钠、安赛蜜在5.0~200 mg/L范围内,标准曲线的线性相关系数分别为0.9997,0.9996和0.9997。其检出限分别为0.37μg/ml,0.12μg/ml和0.26μg/ml,峰面积相对标准差分别为4.0%,1.6%和4.1%。方法平均加标回收率分别为92.1%~111%,88.3%~111%和83.3%~115%。结论该方法快速灵敏,操作简单,适合于饮料中该3种甜味剂的同时检测。
Objective To establish a capillary electrophoresis-UV method for the determination of aspartame, sodium saccharin and acesulfame potassium in beverages. Methods Samples were degassed by ultrasound, centrifuged at 12 000r / min for 5 min, the supernatant was injected directly into the analysis. The fused silica capillary tube (50 cm × 50 μm, effective length 41 cm, uncoated) was used as the separation column. 20 mmol / L borax solution (pH = 9.3 and 15% acetonitrile) With a height of 20 cm and a separation voltage of 15 kV, aspartame, sodium saccharin and acesulfame were baseline separated within 12 min at a wavelength of 210 nm. Results Aspartame, sodium saccharin, and acesulfame potassium in the range of 5.0 ~ 200 mg / L, the linear correlation coefficients of the standard curve were 0.9997, 0.9996 and 0.9997, respectively. The detection limits were 0.37μg / ml, 0.12μg / ml and 0.26μg / ml respectively. The relative standard deviations of peak area were 4.0%, 1.6% and 4.1% respectively. The average recoveries of the method were 92.1% ~ 111%, 88.3% ~ 111% and 83.3% ~ 115%, respectively. Conclusion The method is fast, sensitive and easy to operate. It is suitable for the simultaneous detection of the three sweeteners in beverages.