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目的 探讨 p5 3基因突变在乳腺癌发生发展中的演变规律及其与预后的关系。方法 应用 PCR-单链构象多态性 (PCR- SSCP)分析方法检测 5 5例原发性乳腺癌患者 p5 3基因 5～ 8外显子的突变情况。结果 p5 3基因突变发生的频率为 36 .4% (2 0 / 5 5 ) , ～ 期和 ～ 期乳腺癌中 p5 3基因突变阳性率分别为 47.2 % (17/ 36 )和15 .8% (3/ 19) ,而且突变多发生在 5～ 6外显子。突变阳性患者其无病生存期明显较阴性者差 (P<0 .0 1) ,在 、 期乳腺癌中也以突变阳性患者的预后为差 (P<0 .0 5 )。结论 乳腺癌 p5 3基因突变多发生在病变早期 ,检测该基因的突变有可能成为判断 、 期乳腺癌预后的指标。
Objective To investigate the evolution of p53 gene mutation in the development of breast cancer and its relationship with prognosis. Methods PCR-single strand conformation polymorphism (PCR-SSCP) analysis was used to detect mutations in exons 5-8 of p53 gene in 55 patients with primary breast cancer. Results The frequency of p53 mutations was 36.4% (20/55). The positive rate of p53 mutations in breast cancers in period ~ and period was 47.2% (17/36) and 15.8% ( 3/19), and mutations occur in 5-6 exons. The disease-free survival of patients with positive mutations was significantly worse than that of negative patients (P < 0.01), and the prognosis of patients with positive mutations was also poor (P < 0.05). Conclusion The mutation of p53 gene in breast cancer occurs in early stage of the disease. Detection of the mutation in this gene may be an indicator of prognosis and prognosis of stage breast cancer.