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目的:建立HPLC-ELSD法同时测定急性子中凤仙萜四醇苷A、B、C、D含量的方法。方法:采用反相高效液相色谱-蒸发光散射检测法,使用AlltimaTM-C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水,梯度洗脱,流速0.8 mL/min,柱温35℃。ELSD检测器条件:载气(空气)流速2.6 L/min,漂移管温度95℃。结果:凤仙萜四醇苷A、B、C、D进样量分别在1.250～12.500μg、9.100～91.000μg、10.125～101.250μg、1.625～16.250μg范围内线性关系良好,相关系数(n=6)分别为0.9998、0.9992、0.9993、0.9998;平均回收率(n=6)分别为98.98%、102.50%、98.31%和97.69%,RSD分别为2.79%、1.37%、2.58%和2.45%。结论:本方法简便、灵敏、准确,可用于急性子药材中凤仙萜四醇苷A、B、C、D 4种成分的含量测定。
Objective: To establish a HPLC-ELSD method for the simultaneous determination of porphyritolide tetrandontolide A, B, C and D in acute cases. METHODS: AlltimaTM-C18 column (250 mm × 4.6 mm, 5 μm) was used with reversed-phase high performance liquid chromatography coupled with evaporative light scattering. The mobile phase consisted of methanol and water with a gradient of 0.8 mL / Temperature 35 ℃. ELSD detector conditions: Carrier gas (air) flow rate 2.6 L / min, drift tube temperature 95 ° C. Results: The calibration curves of A, B, C and D of phlebita were linear in the range of 1.250 ~ 12.500μg, 9.100 ~ 91.000μg, 10.125 ~ 101.250μg and 1.625 ~ 16.250μg, respectively. The correlation coefficients (n = 6) were 0.9998,0.9992,0.9993,0.9998 respectively. The average recoveries (n = 6) were 98.98%, 102.50%, 98.31% and 97.69%, respectively, with RSDs of 2.79%, 1.37%, 2.58% and 2.45%, respectively. Conclusion: The method is simple, sensitive and accurate and can be used for the determination of four components of Auxinoside A, B, C and D in acute herbs.