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目的探讨局部晚期宫颈癌(LACC)组织中ERCC1 mRNA及P53蛋白表达与新辅助化疗(NACT)敏感性及预后的关系。方法采集60例病例,在NACT前分别应用RT—PCR(逆转录聚合酶链反应)技术及免疫组化SP方法检测ERCC1mRNA和P53蛋白的表达。结果 60例患者中,有效45例,有效率为75.00%。患者年龄、病理分级及类型与NACT疗效差异均无统计学意义(P﹥0.05)。局部晚期宫颈癌组织中ERCC1mRNA、P53蛋白的表达与NACT疗效有关(P<0.05)。其中ERCC1mRNA及P53过表达均为NACT疗效的危险因素,分别[β=-1.398OR(95%CI)=0.247(0.069~0.880)],[β=-1.981,OR(95%CI)=1.375(0.903~1.519)]。术后病理结果提示具有放疗高危因素者共31例(68.9%)。二者过表达均可提示术后病理结果具有放疗高危因素(P<0.05)。且二者表达水平呈显著正相关(r=0.275,P<0.05)。结论 LACC组织中ERCC1mRNA及P53表达与NACT敏感性相关,并且可提示术后病理结果具有放疗高危因素,两者表达呈正相关。
Objective To investigate the relationship between the expression of ERCC1 mRNA and P53 protein and the sensitivity and prognosis of neoadjuvant chemotherapy (NACT) in locally advanced cervical cancer (LACC). Methods Sixty cases were collected and the expressions of ERCC1 mRNA and P53 protein were detected by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry (SP) respectively before NACT. Results Among the 60 patients, 45 were effective and the effective rate was 75.00%. There was no significant difference in the age, pathological grade and type of patients with NACT (P> 0.05). The expression of ERCC1mRNA and P53 protein in locally advanced cervical cancer correlated with the efficacy of NACT (P <0.05). The ERCC1mRNA and P53 overexpression were risk factors for the NACT response, with β = -1.398OR (95% CI) = 0.247 (0.069-0.880), β = -1.981, OR (95% CI) = 1.375 0.903 ~ 1.519)]. Postoperative pathological findings suggest that 31 patients (68.9%) had high risk of radiotherapy. Both overexpression may be prompted postoperative pathological findings with radiotherapy risk factors (P <0.05). There was a significant positive correlation between the two expression levels (r = 0.275, P <0.05). Conclusion The expression of ERCC1mRNA and P53 in LACC tissues is correlated with the sensitivity of NACT and may indicate that the postoperative pathological findings have high risk of radiotherapy, and the expression of ERCC1mRNA and P53 are positively correlated.