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目的 :研究牙龈卟啉单胞菌在牙周炎患者病变部位和健康部位龈下菌班中的分布情况。方法 :选择6 4例成年牙周炎患者 ,取龈下菌斑 ,经厌氧培养 ,挑取产黑色素菌落 ,经多聚酶链反应鉴定牙龈卟啉单胞菌。结果 :产黑色素 G 厌氧杆菌和牙龈卟啉单胞菌的患者检出率分别是 6 7.2 %和 6 0 .9%。牙龈卟啉单胞菌在病变部位和健康部位的检出率分别是 35 .9%和 2 8.1% ,二者差异无统计学意义 ;牙龈卟啉单胞菌在病变部位和健康部位的检出株数分别是 12 2株和 85株 ,占产黑色素 G 厌氧杆菌检出株数的 32 .3%和 35 .9% ,差异无统计学意义。结论 :牙龈卟啉单胞菌可能作为内源性致病菌在特定条件下过度生长而导致牙周破坏。
Objective: To study the distribution of Porphyromonas gingivalis in the diseased and healthy subgingival bacteria in patients with periodontitis. Methods: Sixty-four adult patients with periodontitis were selected. The subgingival plaque was taken and anaerobically cultured. The melanin-producing colonies were picked and the P. gingivalis was identified by polymerase chain reaction. Results: The detection rates of patients with melanin A and Pseudomonas gingivalis were 62.2% and 60.9%, respectively. The detection rates of Porphyromonas gingivalis in the diseased and healthy parts were 35.9% and 21.8%, respectively, with no significant difference between the two groups. The detection of Porphyromonas gingivalis in diseased and healthy parts The number of strains was 12 2 and 85 respectively, accounting for 32.3% and 35.9% of the detected strains of melanin-producing anaerobe. The difference was not statistically significant. Conclusion: Porphyromonas gingivalis may be an endogenous pathogen that overgrows under certain conditions and causes periodontal destruction.