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以2-羰基丙酸水杨酰腙作为配体与六水硝酸锌在无水乙醇中进行反应,制得2-羰基丙酸水杨酰腙锌(Ⅱ)配合物,经元素分析、红外光谱和乙二胺四乙酸(EDTA)滴定等技术进行了表征。在模拟人体生理条件下,采用荧光光谱和紫外光谱,结合盐效应实验研究了2-羰基丙酸水杨酰腙锌(Ⅱ)配合物与小牛胸腺DNA(ctDNA)的相互作用。研究结果表明2-羰基丙酸水杨酰腙锌(Ⅱ)配合物对溴化乙锭小牛胸腺DNA(EB-ctDNA)体系的猝灭属于静态猝灭;根据修正的SternVolmer方程计算了不同温度下两者的结合常数,并结合Van’t Hoff方程计算出相应的热力学参数,焓变值(ΔH=-34.07kJ/mol)和熵变值(ΔS=-50.91J·mol~(-1)·K~(-1))表明氢键和范德瓦尔斯力是维持2-羰基丙酸水杨酰腙锌(Ⅱ)配合物-EB-ctDNA复合物稳定的主要作用力;2-羰基丙酸水杨酰腙锌(Ⅱ)配合物与ctDNA主要作用方式为嵌插作用,而不是沟槽作用和静电作用。
2-carbonyl propionic acid salicyloyl hydrazone as ligand and zinc nitrate hexahydrate in anhydrous ethanol to obtain 2- carbonyl propionic acid salicyloyl hydrazone zinc (Ⅱ) complex, by elemental analysis, infrared spectroscopy and ethylenediamine Tetraacetic acid (EDTA) titration and other techniques were characterized. Under simulated human physiological conditions, the interaction between 2-oxo-hydrazone hydrazone (Ⅱ) complex and calf thymus DNA (ctDNA) was studied by fluorescence and ultraviolet spectroscopy combined with salt effect. The results show that quenching of EB-ctDNA by 2-carbonylpropionate salicylate hydrazone zinc (Ⅱ) complex belongs to static quenching. According to the modified SternVolmer equation, The thermodynamic parameters, enthalpy change (ΔH = -34.07 kJ / mol) and entropy change (ΔS = -50.91 J · mol -1) · K were calculated according to Van’t Hoff equation. ~ (-1)) showed that the hydrogen bond and van der Waals forces are the main forces to maintain the stability of the zinc (Ⅱ) salicylate hydrazone (Ⅱ) complex-EB-ctDNA complex; 2- (Ⅱ) complexes and ctDNA the main mode of action for intercalation, rather than the role of grooves and electrostatic interactions.