Prevalence of occult hepatitis B virus infection in haemodialysis patients from central Greece

• 作 者: Paraskevi Mina Sarah P Georgiadou Christos Rizos George N Dalekos Eirini I Rigopoulou
• 机 构: Department of Medicine and Research Laboratory of Internal Medicine,Medical School, University of Th
• 出 处: World Journal of Gastroenterology
• 发表日期: 2010年02期
• 关键词: occult DNA prevalence dialysis immunosorbent attending commercially associate re

AIM:To assess the hepatitis B virus(HBV)-DNA and the prevalence of occult HBV infection in end-stage renal failure(ESRF)patients from Central Greece. METHODS:Sera from 366 ESRF patients attending five out of six dialysis units from Central Greece were investigated for HBV-DNA by real-time polymerase chain reaction.Only serum samples with repeatedly detectable HBV-DNA were considered positive.IgG antibodies to hepatitis C virus(anti-HCV)were tested by a third generation enzyme linked immunosorbent assay(ELISA),while IgG antibodies to hepatitis E virus (anti-HEV)were tested by two commercially available ELISAs.RESULTS:HBV-DNA was detected in 15/366 patient (4.1%)and HBsAg in 20/366(5.5%).The prevalenc of occult HBV infection was 0.9%(3/346 HBsAg negative patients).Occult HBV was not associate with a specific marker of HBV infection or anti-HCV o anti-HEV reactivity.There was no significant differenc in HBV-DNA titres,demographic and biochemica features,between patients with occult HBV infectio and those with HBsAg-positive chronic HBV infection. CONCLUSION:In central Greece,4%of ESRF patient had detectable HBV-DNA,though in this setting,th prevalence of occult HBV seems to be very low(0.9%).

AIM: To assess the hepatitis B virus (HBV) -DNA and the prevalence of occult HBV infection in end-stage renal failure (ESRF) patients from Central Greece. METHODS: Sera from 366 ESRF patients attending five out of six dialysis units from Central Greece were investigated for HBV-DNA by real-time polymerase chain reaction. Both serum samples with repeated detectable HBV-DNA were considered positive. IgG antibodies to hepatitis C virus (anti-HCV) were tested by a third generation enzyme linked immunosorbent assay ELISA) while IgG antibodies to hepatitis E virus (anti-HEV) were tested by two commercially available ELISAs. RESULTS: HBV-DNA was detected in 15/366 patient (4.1%) and HBsAg in 20/366 (5.5%). The prevalence of occult HBV infection was 0.9% (3/346 HBsAg negative patients). Occult HBV was not associate with a specific marker of HBV infection or anti-HCV o anti-HEV reactivity. There was no significant differenc in HBV-DNA titres , demographic and biochemica features, patients with occult HBV in CONCLUSION: In central Greece, 4% of ESRF patient had detectable HBV-DNA, though in this setting, th prevalence of occult HBV seems to be very low (0.9%).