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目的:基于纳米金对鲁米诺-氯化汞化学发光体系有良好的催化作用,建立测定木犀草素含量的高灵敏流动注射-化学发光分析法。方法:采用流动注射技术,实验研究了影响化学发光的各种因素;根据体系的化学发光光谱和紫外-可见吸收光谱结果,探讨了可能的化学发光机理。结果:0.1 mol·L-1氢氧化钠、4.0×10-4 mol·L-1鲁米诺、6.08×10-5 mol·L-1纳米金与1.0×10-3 mol·L-1 HgCl2组成最优的化学发光体系。在优化的实验条件下,本方法测定木犀草素的线性范围为2.0×10-9～1.0×10-7 g·mL-1(r=0.9929),检出限为1.5×10-9 g·mL-1,RSD为2.1%(C=1.0×10-8 g·mL-1,n=11)。纳米金作为电子转移的媒介,在鲁米诺-氯化汞反应过程中起催化作用;木犀草素和鲁米诺竞争与氯化汞发生反应,抑制了鲁米诺氧化成鲁米诺自由基,导致发光强度的降低。结论:利用本法成功地测定了血清与合成样品中木犀草素的含量。
OBJECTIVE: To establish a sensitive flow-injection chemiluminescence assay for the determination of luteolin in a luminol-mercuric chloride chemiluminescence system based on gold nanoparticles. Methods: Flow injection technique was used to study the various factors affecting chemiluminescence. Based on the chemiluminescence and UV-Vis absorption spectra of the system, possible chemiluminescence mechanisms were discussed. Results: 0.1 mol·L-1 sodium hydroxide, 4.0 × 10-4 mol·L-1 luminol, 6.08 × 10-5 mol·L-1 nano gold and 1.0 × 10-3 mol·L-1 HgCl2 Composition of the best chemiluminescence system. Under the optimal experimental conditions, the linear range of luteolin was 2.0 × 10-9 ~ 1.0 × 10-7 g · mL-1 (r = 0.9929) and the detection limit was 1.5 × 10-9 g · mL-1 with RSD of 2.1% (C = 1.0 × 10-8 g · mL-1, n = 11). Nano gold as a catalyst for electron transfer plays a catalytic role in the reaction of luminol with mercuric chloride. The competition between luteolin and luminol reacts with mercuric chloride, which inhibits the oxidation of luminol into luminol , Resulting in a decrease in luminous intensity. Conclusion: This method was successfully used to determine the content of luteolin in serum and synthetic samples.