利用种子蛋白SDS-PAGE电泳谱带鉴定菜豆品种真实性与纯度的研究

• 作 者: 张雪 李爽 韩玉珠
• 机 构: 吉林农业大学园艺学院,吉林省农业科学院农村能源研究所,
• 出 处: 北方园艺
• 发表日期: 2014年07期
• 关键词: 菜豆品种 电泳谱带 纯度 贮藏蛋白 电泳图谱 品种真实性 技术鉴定 电泳方法 蛋白质组分 分子标记鉴定

以不同采收年限的17个菜豆品种的种子为试材,采用SDS-PAGE电泳方法获得菜豆种子贮藏蛋白的电泳图谱,比较分析其真实性;同时研究种子蛋白SDS-PAGE技术鉴定菜豆品种真实性和纯度的可行性。结果表明:17个菜豆品种可以通过SDS-PAGE方法得到特征谱带A、B、C、D、E、F、G、H,且各品种谱带差异明显;SDS-PAGE蛋白质电泳方法用于鉴定菜豆品种真实性和种子纯度是可行的。

Electrophoregram of the storage protein of bean seeds was obtained by SDS-PAGE electrophoresis. The authenticity of the storage protein was also analyzed. The authenticity of the bean varieties was also studied by SDS-PAGE And the purity of the feasibility. The results showed that the characteristic bands A, B, C, D, E, F, G and H of the 17 kidney bean cultivars could be identified by SDS-PAGE, and the bands of different varieties were significantly different. SDS-PAGE electrophoresis was used to identify Bean authenticity and seed purity is feasible.