论文部分内容阅读
目的:观察ω-3多不饱和脂肪酸(ω-3 Polyunsaturated fatty acid,ω-3 PUFA)对人前列腺癌PC-3细胞和乳腺癌MDA-MB-231细胞Rho蛋白翻译后修饰的影响。方法:60μmol/L的二十碳五烯酸(eicosapentaenoic acid,EPA)和二十二碳六烯酸(docosahex-aenoic acid,DHA)处理PC-3和MDA-MB-231细胞24h后,检测EPA和DHA对法尼基蛋白转移酶活性的影响,对Rho蛋白的法尼基化修饰的影响,对Rho蛋白与GTP结合能力的影响。结果:EPA及DHA均能显著下调PC-3和MDA-MB-231细胞法尼基蛋白转移酶活性(P<0.01),抑制Rho蛋白(RhoA、Rac1、Rac2和Cdc42)的法尼基化修饰(P<0.01),并降低PC-3细胞Rho蛋白(RhoA、Rac1和Cdc42)与GTP的结合能力(P<0.05)。结论:ω-3 PUFA可能通过抑制肿瘤细胞Rho蛋白翻译后修饰,而影响肿瘤细胞的生物学特性。
Objective: To investigate the effect of ω-3 polyunsaturated fatty acid (ω-3 PUFA) on the post-translational modification of Rho protein in human prostate cancer PC-3 cells and breast cancer MDA-MB-231 cells. METHODS: PC-3 and MDA-MB-231 cells were treated with 60 μmol / L eicosapentaenoic acid (EPA) and docosahex-aenoic acid (DHA) And DHA on farnesyl protein transferase activity, the impact of Rho protein farnesylation, Rho protein and GTP binding capacity. RESULTS: Both EPA and DHA significantly down-regulated farnesyl protein transferase activity in PC-3 and MDA-MB-231 cells (P <0.01) and inhibited farnesylation of Rho proteins (RhoA, Rac1, Rac2 and Cdc42) (P <0.01), and decreased the binding ability of Rho proteins (RhoA, Rac1 and Cdc42) to GTP in PC-3 cells (P <0.05). CONCLUSION: Omega-3 PUFA may affect the biological characteristics of tumor cells by inhibiting the post-translational modification of Rho protein in tumor cells.