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目的 测定五种 β 内酰胺酶抑制剂复合制剂对产不同型别的超广谱 β 内酰胺酶 (ES BLs)菌株的抗菌活性。方法 用NCCLS推荐的纸片扩散法测定产不同型别ESBLs菌株的敏感性 ,使用聚合酶链反应 (PCR)测定ESBLs的型别。结果 15 4例产ESBLs菌株对哌拉西林 他唑巴坦 (PIP)、头孢哌酮 舒巴坦 (SPZ)、阿莫西林 克拉维酸 (AOA)、替卡西林 克拉维酸 (TIA)和氨苄西林 舒巴坦 (AMS)的敏感率分别是 95 .5 %、38.3%、7.14%、3.2 %和 1.3% ,产TEM及SHV型ESBLs菌株对PIP的敏感率分别是 10 0 %和 89.7% ,对SPZ的敏感率分别为 39.4%和 2 7.6 %。抗菌活性有极显著差异 (P <0 .0 0 5 )。PIP和SPZ对产TEM和SHV型ESBLs菌株的抗菌活性无明显区别 (P >0 .0 5 )。结论 PIP对产ESBLs菌株的抗菌活性明显强于SPZ、AOA、TIA以及AMS等其它酶抑制剂复合制剂
Objective To determine the antibacterial activity of five β-lactamase inhibitors against different types of extended-spectrum β-lactamase (ESBLs) strains. Methods The sensitivity of different strains of ESBLs producing strains was determined by disk diffusion method recommended by NCCLS. The types of ESBLs were determined by polymerase chain reaction (PCR). Results A total of 15 4 ESBLs-producing strains were tested for efficacy against piperacillin-tazobactam (PIP), cefoperazone-sulbactam (SPZ), amoxicillin-clavulanic acid (AOA), ticarcillin- The susceptibilities to AMS were 95.5%, 38.3%, 7.14%, 3.2% and 1.3%, respectively. The sensitivity of TEM and SHV ESBLs isolates to PIP was 100% and 89.7%, respectively, Sensitivity to SPZ was 39.4% and 22.6%, respectively. The antibacterial activity had extremely significant difference (P <0.05). The antibacterial activities of PIP and SPZ against TEM and SHV ESBLs strains were not significantly different (P> 0.05). Conclusion The antibacterial activity of PIP against ESBLs-producing strains was significantly stronger than that of other enzyme inhibitors such as SPZ, AOA, TIA and AMS