论文部分内容阅读
枯草芽孢杆菌Ｂ－９０３菌株是从郑州果园中筛选出的，它代谢产生的抗菌物质对多种植物病原菌具有强抑制作用。通过对其抗菌物质产生条件的研究认为，在ＮＹＤＡ培养液中，培养液初始ｐＨ值为７时，最有利于抗菌物质的产生；振荡培养１１２ｈ，培养滤波的抑菌活性可达最大值；不同初始接菌量对抗菌物质的产生时间影响不大；在２５０ｍｌ三角瓶中，随装液量减少，培养液抑菌活性有增大趋势；大米粉和豆饼粉是最适合产生抗菌物质的碳、氮源；调节培养滤液ｐＨ值至４为抑菌活性物质最简便的保存方法，振荡培养１１２ｈ的培养滤液的最低抑菌浓度为稀释２３１８倍。
The Bacillus subtilis B-903 strain was screened from Zhengzhou orchards. The antibacterial substances produced by its metabolism have a strong inhibitory effect on many plant pathogenic bacteria. According to the research on the conditions of antibacterial production, the antibacterial substances are most conducive to the production of antibacterial substances in the culture medium of NYDA when the initial pH value is 7; shaking culture for 112h, the maximum inhibitory activity of culture filter up to the maximum; The initial amount of inoculant had little effect on the antimicrobial substance production time. In the 250ml flask, the antibacterial activity of the culture medium increased with the decrease of the amount of liquid. Rice flour and soybean cake powder were the most suitable carbon, Nitrogen source; adjust the culture filtrate pH to 4 for the most convenient method of preservation of antibacterial substances, shaking culture 112h culture filtrate minimum inhibitory concentration of 2318 times diluted.