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ｍＲＮＡ的定量分析是基因表达调控研究中应用的重要方法．与斑点杂交、ＲＮＡ印迹法相比，用ＲＮａｓｅ保护试验测定ＲＮＡ具有灵敏度高、操作简便等优点．应用ＲＮａｓｅ保护试验成功地对转基因鼠中人βＥ－珠蛋白基因及鼠ａ－珠蛋白基因的表达水平进行了分析
The quantitative analysis of mRNA is an important method used in the study of gene expression regulation. Compared with blot hybridization and Northern blotting, the RNA assay with RNase has the advantages of high sensitivity, easy operation and so on. The RNase protection assay was used to successfully analyze the expression of human βE-globin gene and murine a-globin gene in transgenic mice