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EPO依赖细胞珠在EPO浓度梯度中培养72小时，再加入MTT培养5小时，离心弃上清后，加入有机溶剂溶解MTT代谢产物，以OD值对EPO浓度梯度作量效曲线，定量地测定EPO生物学活性。本法重复性好，与其它体外生物学活性测定方法所测定结果相符。
EPO was cultured in EPO concentration gradient for 72 hours, and then added MTT for 5 hours. The supernatant was centrifuged and dissolved in organic solvent to dissolve MTT metabolites. The OD value of EPO was used as the dose-response curve to quantitatively determine the concentration of EPO Biological activity. The repeatability of this method is good, consistent with the results of other in vitro biological activity assay.