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用ＳＰＭ法分离基因ＣｐＧ岛的实验所获大量阻留片段中，含有很多非ＣｐＧ岛的序列，包括Ａｌｕ重复序列。为了进一步改进ＳＰＭ方法，最大限度驱除非ＣｐＧ岛的影响，对所有ＳＰＭ阻留的ＤＮＡ片段进行热力学及其电泳行为分析。结果显示，所有ＤＮＡ片段均呈现不同形式的部分解链状态，有的ＤＮＡ片段有２个以上的解链区域；根据各解链区域解链温度差异的大小，有的在ＤＮＡ片段的两侧部分解链、有的在片段的中央部分解链，有的既有中间又有两侧的部分解链，而且ＣｐＧ岛的最高解链温度区与非ＣｐＧ岛有明显差异。若能利用这些ＤＮＡ片段解链上的差异，改变实验条件，使ＤＮＡ片段之间的差异充分显示，就能显著提高ＳＰＭ的效率。
A large number of retained fragments obtained by experiments using SPM to isolate CpG islands contained many non-CpG island sequences, including Alu repeats. In order to further improve the SPM method, the influence of non-CpG islands was maximally eliminated, and all the SPM-retained DNA fragments were subjected to thermodynamic and electrophoretic behavior analysis. The results showed that all DNA fragments showed different forms of partial melting state, and some DNA fragments had more than two melting regions. According to the difference in melting temperature of each melting region, some DNA fragments Some of them were unzipped in the central part of the fragment, some of them both unmounted in the middle and the two sides, and the highest melting temperature region of CpG island was significantly different from non-CpG island. If we can take advantage of the differences in melting of these DNA fragments, changing the experimental conditions so that the differences between the DNA fragments are fully displayed, the efficiency of the SPM can be significantly improved.