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目的 观察大鼠心肌缺血前给予含不同浓度的L-精氨酸和L-NAME的KH灌注液的心肌保护作用。方法 在心肌缺血前给予含不同浓度的L-精氨酸和L-NAME的KH灌注液,再灌注期间测定心脏功能指标及冠脉流出液中心肌酶释放量,观察心肌超微结构改变。结果 心肌缺血前给予含低浓度L-精氨酸的KH液(10 m m ol/L)灌注心脏,能明显减轻心肌缺血再灌注损伤,心肌缺血前给予含高浓度L-精氨酸的KH 液(100 m m ol/L)灌注心脏明显加重心肌缺血再灌注损伤,而给予含L-NAME的KH液无明显心肌保护或损害作用。结论 L-arg-NO 途径在心肌缺血再灌注损伤中有双重作用,既有有益一面,又有有害一面,其利弊取决于两者之间的平衡或净效应
Objective To observe the myocardial protective effect of KH perfusion solution containing different concentrations of L-arginine and L-NAME before myocardial ischemia in rats. Methods KH perfusate with different concentrations of L-arginine and L-NAME was given before myocardial ischemia. During reperfusion, cardiac function index and myocardial enzyme release were measured. Myocardial ultrastructural changes were observed. Results Before myocardial ischemia, KH solution (10 mmol / L) with low concentration of L-arginine was perfused into the heart, which could significantly reduce myocardial ischemia-reperfusion injury. Before myocardial ischemia, L-arginine KH solution (100 mm ol / L) significantly increased myocardial ischemia-reperfusion injury, whereas KH solution containing L-NAME showed no significant myocardial protection or impairment. Conclusions The L-arg-NO pathway has a dual role in myocardial ischemia-reperfusion injury, both beneficial and harmful. The advantages and disadvantages depend on the balance or net effect between the two