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目的:比较生物膜状态与浮游状态下粪肠球菌对碱的耐受性。方法:制备浮游状态和生物膜状态的粪肠球菌细胞,用pH值分别为7、8、9、10、11和12的培养液作用2h,利用MTT比色法比较2种状态下细菌细胞活性变化。采用SAS6.12软件包对数据进行统计学分析。结果:低碱性环境(pH值7～9)对粪肠球菌的生长无明显影响,高碱性环境(pH值>10)下存活细菌的比例明显减少;高pH环境下,生物膜状态的粪肠球菌存活细菌比例显著高于浮游状态下细菌的存活比例。结论:粪肠球菌对碱性环境具有强耐受性,形成生物膜是粪肠球菌抵抗高碱性环境的一个重要原因。
Objective: To compare the tolerance of alkali-tolerant Enterococcus faecalis to biofilm status and planktonic status. Methods: The Enterococcus faecalis cells in the floating state and the biofilm state were prepared. The culture medium of pH 7, 8, 9, 10, 11 and 12 was used for 2 hours. The bacterial cell activity Variety. SAS6.12 software package for statistical analysis of the data. Results: The growth of Enterococcus faecalis was not affected in low alkalinity (pH value of 7-9), and the proportion of viable bacteria in over-alkaline environment (pH> 10) decreased significantly. Under high pH environment, the biofilm state The proportion of viable bacteria of Enterococcus faecalis was significantly higher than that of bacteria in the floating state. Conclusion: Enterococcus faecalis is highly tolerant to alkaline environment and biofilm formation is an important reason for Enterococcus faecalis to resist over-alkaline environment.