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目的 建立高效液相色谱法测定蜂丁麻滴眼剂中盐酸麻黄碱含量的方法。 方法 采用YWGC18分析色谱柱 ( 4 6mmID× 2 5 0mm ,粒径 10 μm) ,C18保护柱 ( 4 6mmID× 5mm ,粒径 10 μm) ,流动相 10 %乙腈 ( 0 5 %三乙胺 ,磷酸调pH3 0 ) ,流速 1 0ml/min ,检测波长 2 0 8nm ,测定对照品和样品色谱图 ,记录盐酸麻黄碱色谱峰面积 ,计算其含量。 结果 盐酸麻黄碱的理论板数为 10 0 0 0。回归方程 :y =90 5 878+ 16 15 1738x ,r=0 9999,线性范围 0 4 0 32～ 1 6 13μg。平均回收率为 99 2 % (RSD1 6 % )。 结论 该法操作简便 ,结果准确 ,可用于测定蜂丁麻滴眼剂中盐酸麻黄碱的含量
Objective To establish a method for the determination of ephedrine hydrochloride in bee jing ophthalmic solution by high performance liquid chromatography. Methods The chromatographic column (4.6 mmID × 250 mm, particle size 10 μm), C18 guard column (4.6 mmID × 5 mm, particle size 10 μm), mobile phase of 10% acetonitrile (0 5% pH3 0), flow rate 10ml / min, detection wavelength 280nm, the determination of reference substance and sample chromatograms, recording peak area of ephedrine hydrochloride, calculate its content. Results The theoretical plate number of ephedrine hydrochloride was 10 000. Regression equation: y = 90 5 878 + 16 15 1738x, r = 0 9999, linear range 0 4 0 32 ~ 1 6 13μg. The average recovery was 99 2% (RSD 16%). Conclusion The method is simple, accurate and can be used to determine the content of ephedrine hydrochloride in bee jing ophthalmic solution