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随着BtCry作物在我国的广泛应用和推广,靶标害虫对其抗性风险已成为BtCry作物生态安全研究的重要内容。氨肽酶N(Aminopeptidase N,APN)是位于昆虫中肠刷状缘膜囊泡(Brush Border Membrane Vesicles,BBMV)上BtCry毒素重要的受体蛋白之一,它与BtCry毒素的结合能力决定了BtCry毒素的杀虫活性及昆虫对Bt抗性的产生。本文从APN的结构特征与分类、APN与BtCry毒素的结合特异性、结合位点、结合过程中的分子互作机制及APN变异导致昆虫抗性产生几方面系统综述了鳞翅目昆虫中肠BtCry受体蛋白-氨肽酶N与BtCry毒素的结合及其与Bt抗性关系的研究进展。
With the widespread application and promotion of BtCry crops in China, the risk of target pests to its resistance has become an important part of the research on ecological security of BtCry crops. Aminopeptidase N (APN) is one of the most important receptor proteins of BtCry toxin located in the brush border membrane barrier (BBMV) of insects and its binding ability to BtCry toxin determines BtCry Insecticidal Activity of Toxins and Insect Resistance to Bt Production. In this paper, the structural and taxonomic characteristics of APN, the binding specificity of APN and BtCry toxin, the binding sites, the molecular interaction mechanism during the binding process and the APN variation resulted in several aspects of insect resistance. Binding of Receptor Protein Aminopeptidase N to BtCry Toxin and Its Relationship with Bt Resistance.