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将小麦花药培养愈伤组织转入含有不同浓度秋水仙素的C17培养基中,进行幼苗分化和染色体加倍。结果表明,5mg/L秋水仙素能获得较好的加倍效果,加倍率为83.33%,比常规加倍法提高30.95个百分点,但对幼苗分化率无明显影响。
Wheat anther culture callus was transferred to C17 medium containing different concentrations of colchicine for seedling differentiation and chromosome doubling. The results showed that 5mg / L colchicine could achieve better doubling effect with the doubling rate of 83.33%, which was 30.95% higher than that of conventional doubling method, but had no obvious effect on the seedling differentiation rate.