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利用抗体的双价性交联两种抗原物质的概念并不新颖。用化学重组实验已制成杂交的抗体分子,并成功地用于组织化学以铁蛋白显示抗原位点。但因难以取得这样的杂交抗体,以致此法很少使用。其他免疫酶技术如“夹心法”或酶抗体法的应用已日益普及,后者需要纯化的抗体,而未标记酶法由于两种IgG抗体可用第三种抗体加以交联,因此毋需提纯有关的抗体。随着单克隆抗体
The idea of using bi-valent antibodies to cross-link two antigens is not new. Hybridized antibody molecules have been made using chemical recombination experiments and have been successfully used in histochemistry to show antigenic sites with ferritin. However, this method is rarely used because it is difficult to obtain such a hybrid antibody. The use of other immunoenzyme techniques such as “sandwich” or enzyme-antibody methods has become increasingly popular, and the latter requires purified antibodies, whereas unlabeled enzymatic methods require no purification as the two IgG antibodies can be cross-linked with a third antibody Antibody. With monoclonal antibodies