【摘 要】
：
Apical actin filaments are highly dynamic structures that are crucial for rapid pollen tube growth,but the mechanisms regulating their dynamics and spatial organization remain incompletely understood.We here identify that AtAIP1-1 is important for regulat
【机 构】
：
Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China;Center for Plant Biology,School
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Apical actin filaments are highly dynamic structures that are crucial for rapid pollen tube growth,but the mechanisms regulating their dynamics and spatial organization remain incompletely understood.We here identify that AtAIP1-1 is important for regulating the turnover and organization of apical actin filaments in pollen tubes.AtAIP1-1 is distributed uniformly in the pollen tube and loss of function of AtAIP1-1 affects the organization of the actin cytoskeleton in the pollen tube.Specifically,actin filaments became disorganized within the apical region of aip1-1 pollen tubes.Consistent with the role of apical actin filaments in spatially restricting vesicles in pollen tubes,the apical region occupied by vesicles becomes enlarged in aip1-1 pollen tubes compared to WT.Using ADF1 as a representative actin-depolymerizing factor,we demonstrate that AtAIP1-1 enhances ADF1-mediated actin depolymerization and filament severing in vitro,although AtAIP1-1 alone does not have an obvious effect on actin assembly and disassembly.The dynamics of apical actin filaments are reduced in aip1-1 pollen tubes compared to WT.Our study suggests that AtAIP1-1 works together with ADF to act as a module in regulating the dynamics of apical actin filaments to facilitate the construction of the unique“apical actin structure”in the pollen tube.
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