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目的:探讨与肿瘤生长浸润和转移相关的表皮生长因子受体(HER-2)、基质金属蛋白酶-2(MMP-2)、胰岛素样生长因子-1(IGF-1)和血管内皮生长因子(VEGF)在新疆维吾尔族妇女宫颈癌组织中的表达及意义。方法:收集维吾尔族妇女浸润性宫颈鳞癌患者的宫颈癌组织38例,并收集正常宫颈组织32例作为正常对照。分别运用实时荧光定量PCR(Q-PCR)和蛋白免疫印迹(Western blot)技术检测HER-2、MMP-2、IGF-1和VEGF在不同宫颈组织中的m RNA及蛋白表达水平。结果:Q-PCR检测结果显示,与正常对照组相比,HER-2、IGF-1、MMP-2和VEGF的m RNA表达水平在宫颈组织中均有不同程度的升高,差异均有统计学意义(P<0.05或<0.01)。Western blot检测显示HER-2、MMP-2和VEGF蛋白表达水平在宫颈癌组中的表达亦均较正常对照组有不同程度的升高,差异均有统计学意义(P<0.05或P<0.01),而IGF-1在两组之间的表达差异无统计学意义(P>0.05)。结论:HER-2、MMP-2、IGF-1和VEGF在维吾尔族妇女宫颈癌组织中的过度表达可能与宫颈癌的发生密切相关。
OBJECTIVE: To investigate the expression of HER-2, MMP-2, IGF-1 and VEGF in tumor infiltration and metastasis Expression of VEGF in Cervical Cancer of Uygur Women in Xinjiang and Its Significance. Methods: Thirty-eight cervical cancer patients with invasive cervical squamous cell carcinoma were collected from Uigur women and 32 normal cervical tissues were collected as normal control. The mRNA and protein levels of HER-2, MMP-2, IGF-1 and VEGF in different cervical tissues were detected by real-time quantitative PCR (Q-PCR) and Western blot respectively. Results: The results of Q-PCR showed that the expression of m RNA of HER-2, IGF-1, MMP-2 and VEGF were all increased to some extent in cervical tissues compared with the normal control group Significance (P <0.05 or <0.01). Western blot showed that the expression of HER-2, MMP-2 and VEGF in cervical cancer group also increased to some extent compared with the normal control group, the difference was statistically significant (P <0.05 or P <0.01 ), While the expression of IGF-1 in the two groups showed no significant difference (P> 0.05). Conclusion: The overexpression of HER-2, MMP-2, IGF-1 and VEGF in Uighur women with cervical cancer may be closely related to the occurrence of cervical cancer.