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方法试验种茎为三叶半夏(Pinellia ter-nate Breit),由南充县药材公司提供。1984年春,将种茎种入南充师院的校园内,当株高10cm 出现大叶时,取幼嫩叶片为外植体进行培养。培养基为MS、B_5附加成分为2,4—D0.2,NAA0.2、KT0.5(单位均为mg/l),PH 为5.8。接种后的试管置于培养室内,温度23～25℃,用40瓦的日光灯,每天光照10小时。接种后6天叶块开始膨大,10天产生愈伤组织,30天左右愈伤组织出现绿点,接着分化出幼苗,两个月左右试管内形成3～4cm 高的完整
Methods The test stems were Pinellia ternata Breit, provided by Nanchong County Medicinal Materials Company. The spring of 1984, the seeds planted in Nanchong Teachers College campus, when the plant height 10cm large leaves, take young leaves as explants for culture. The medium is MS. The additional components of B_5 are 2,4-D0.2, NAA0.2 and KT0.5 (all in mg / l), and the pH is 5.8. The inoculated tubes were placed in a culture chamber at a temperature of 23-25 ° C with a fluorescent lamp of 40 watts per day for 10 hours. Six days after inoculation, the leaves started to expand, callus formed in 10 days, green spots appeared in callus in about 30 days, and then the seedlings were differentiated, and the formation of 3 to 4 cm in length was formed in test tubes in about two months