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目的分析宁夏HIV抗体筛查阳性标本的确证符合情况,了解不同抗体筛查实验室检测状况以及不同人群确证阳性状况,为指导检测工作提供依据。方法对HIV抗体初筛阳性标本,采用酶联免疫吸附试验(ELISA)和胶体硒法进行复检,2种试剂均呈阳性或一阴一阳的用蛋白免疫印迹法(WB)进行确证,确证阳性病例进行流行病学分析。结果384例经复检后阳性标本271例,WB确证196例为HIV-1抗体阳性,确证阳性率为72.32%。确证阳性率随s/co值增大呈上升趋势。从WB带型分布来看,抗env基因编码蛋白抗体、抗pol基因编码抗体、gag基因编码蛋白抗体的平均阳性率分别为99.66%、92.35%、87.24%,检出不确定样本共18份。全年共完成追踪检测5人,其中转为阳性的1人。结论艾滋病各网络实验室应进一步加强实验室规范化管理,尽量消除引起初筛试验假阳性结果的影响因素。同时,加大健康教育和对高危人群行为干预的力度,注意HIV感染老龄化趋势,遏制艾滋病的蔓延。
Objective To analyze the confirmations of positive HIV antibody screening in Ningxia and to find out the status of different antibody screening laboratories and positive confirmations in different populations so as to provide the basis for the guidance of the detection. Methods Positive samples of HIV antibody were screened by enzyme-linked immunosorbent assay (ELISA) and selenium-selenium method. The two reagents were positive or yin-yang were confirmed by Western blotting (WB) Positive cases of epidemiological analysis. Results Among the 384 cases, 271 were positive after retesting, and 196 were confirmed by WB as positive for HIV-1 antibody. The positive rate was 72.32%. The positive rate confirmed with the s / co value increased upward trend. The average positive rates of antibody encoding anti-env gene, anti-pol gene encoding antibody and gag gene encoding antibody were 99.66%, 92.35% and 87.24% respectively, and 18 samples were undetermined. A total of 5 follow-up tests were completed in the year, of which 1 was converted to positive. Conclusion All HIV / AIDS laboratories should further standardize laboratory management and try their best to eliminate the influencing factors of false positive results. At the same time, we should increase health education and intervene in behaviors of high-risk groups, pay attention to the trend of aging HIV infection and curb the spread of AIDS.