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为了有效提高俄罗斯玉米种质资源的利用效率,拓宽黑龙江省玉米自交系的遗传基础,利用SSR分子标记技术研究91份俄罗斯玉米自交系的遗传多样性,并对其进行聚类分析。结果表明,50个多态性SSR标记共检测到248个等位基因,平均4.96个;位点多态性信息指数(PIC)平均为0.67,表明俄罗斯玉米自交系的遗传多样性较为丰富。聚类分析(邻近法)结果将俄罗斯玉米自交系划分为8个类群,类群I占25.27%,兰卡斯特和瑞德群各占20.88%,旅大红骨和塘四平头分别占13.19%和9.89%;类群II、类群III和P群分别占4.40%、3.30%和2.20%。俄罗斯玉米自交系类群I种质资源较为丰富,可能与光温不敏感特性有关。在遗传多样性分析基础上,利用11个SSR标记构建了91份俄罗斯玉米自交系特异分子身份证,为黑龙江省玉米杂种优势利用及育种实践提供了科学依据。
In order to effectively improve the utilization efficiency of Russian maize germplasm resources and broaden the genetic basis of maize inbred lines in Heilongjiang Province, the genetic diversity of 91 Russian maize inbred lines was studied using SSR molecular markers and clustering analysis was conducted. The results showed that a total of 248 alleles were detected in 50 polymorphic SSR markers, with an average of 4.96. The average polymorphic information index (PIC) was 0.67, indicating that the genetic diversity of Russian maize inbred lines was abundant. Clustering Analysis (Adjacent Method) Results The Russian maize inbred lines were divided into 8 groups, with 25.27% in group I, 20.88% in Lancaster and Rhede, 13.19% And 9.89% respectively; Group II, Group III and P accounted for 4.40%, 3.30% and 2.20% respectively. Russia I maize inbreds I germplasm resources are more abundant, may be insensitive to light and temperature characteristics. Based on the analysis of genetic diversity, 91 SSR markers were used to construct 91 molecular ID maize inbred lines of Russian maize, which provided a scientific basis for the utilization and breeding of maize heterosis in Heilongjiang Province.