论文部分内容阅读
目的分析闽北2014年登革热暴发疫情的病因,从分子水平探讨流行毒株的生物学特征,追踪其地域来源。方法采集医院就诊人员中登革热患者血清标本并进行流行病学调查,RT-PCR检测登革病毒核酸,核酸阳性标本进行登革病毒E基因核苷酸序列测定分析。结果采集8份血清标本中,分离到7株登革病毒1型(DENV1)毒株,1株登革病毒2型(DENV2)毒株。根据序列比对和亲缘性系统进化树,编号为S030、S073、S126、S185、S186和S197的登革热1型病毒样本,与亚洲的毒株AY762084.1、EU179860.1、AY732474.1、JQ917404.1、FJ478458.1、KR071622.1、KR052012.1和KP686070.1亲缘关系较近,同源性较高。编号为S078的登革热2型病毒样本,与亚洲的毒株EU081178.1,EF051521.1,DQ645556.1,KP012546.1亲缘关系接近,其中与广东的毒株KP012546.1最近,同源性较高。结论DENV1的毒株与亚洲中国中原1型的毒株同源性较高,推测这些样本均由亚洲地区输入到中国,并在中国中原湖北、河南以及福建分散流行。DENV2的毒株由广东输入到闽北地区。
Objective To analyze the etiopathogenisis of the outbreak of dengue fever in northern Fujian in 2014 and to explore the biological characteristics of the epidemic strain at the molecular level and trace its geographical origin. Methods Serological samples from dengue fever patients in hospital were collected for epidemiological investigation. The dengue virus nucleic acid and nucleic acid positive samples were detected by reverse transcription polymerase chain reaction (RT-PCR). Results Eight strains of sera from dengue virus type 1 (DENV1) and one strain of dengue virus type 2 (DENV2) were isolated. According to the sequence alignment and phylogenetic tree phylogenetic tree, the dengue virus type 1 viruses numbered S030, S073, S126, S185, S186 and S197 were compared with the Asian strains AY762084.1, EU179860.1, AY732474.1, JQ917404. 1, FJ478458.1, KR071622.1, KR052012.1 and KP686070.1 are closely related and have high homology. The dengue 2 virus sample numbered S078 was closely related to the Asian isolates EU081178.1, EF051521.1, DQ645556.1 and KP012546.1, with the closest homology to Guangdong strain KP012546.1 . Conclusions The strains of DENV1 have high homology with the strains of Central China 1 in Asia. It is speculated that these samples were imported into China from Asia and dispersed in Hubei, Henan and Fujian in China. The strain of DENV2 was imported from Guangdong to northern Fujian.