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目的:建立同时测定酒花提取物中芸香苷、紫云英苷、槲皮素和黄腐酚4个黄酮成分含量的UPLC法。方法:采用Hypersil BDS C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-1%甲酸水溶液为流动相,梯度洗脱,体积流量0.8 mL·min-1,柱温25℃,检测波长为350 nm。结果:芸香苷、紫云英苷、槲皮素和黄腐酚分别在0.011～0.187μg、0.003～0.054μg、0.011～0.194μg和0.016～0.265μg范围内线性关系良好,相关系数(r)分别为0.9978、0.9968、0.9998和0.9999,平均加样回收率分别为99.5%、103.7%、101.6%和101.7%,RSD分别为2.2%、1.5%、2.2%和2.4%。酒花黄酮提取物中芸香苷、紫云英苷、槲皮素和黄腐酚的含量分别为4.57、0.60、1.03和13.28 mg·g-1。结论:该方法简便快速,准确可靠,重复性好,可用于酒花药材和提取物的质量评价。
OBJECTIVE: To establish a UPLC method for the simultaneous determination of rutoside, orientin, quercetin and xanthohumol in hops extracts. Methods: Hypersil BDS C18 column (4.6 mm × 250 mm, 5 μm) was used with mobile phase of acetonitrile-1% formic acid as mobile phase. The volume flow rate was 0.8 mL · min-1 and the column temperature was 25 ℃. The detection wavelength was 350 nm. Results: Rutin, astragalin, quercetin and xanthohumol had good linearity in the range of 0.011 ~ 0.187μg, 0.003 ~ 0.054μg, 0.011 ~ 0.194μg and 0.016 ~ 0.265μg, respectively. The correlation coefficient The average recoveries were 99.5%, 103.7%, 101.6% and 101.7%, respectively, with RSDs of 2.2%, 1.5%, 2.2% and 2.4%, respectively. The contents of rutin, astragalin, quercetin and xanthohumol in the extracts of hop flavones were 4.57, 0.60, 1.03 and 13.28 mg · g-1, respectively. Conclusion: The method is simple and rapid, accurate and reliable, reproducible, and can be used for the quality evaluation of hops and extracts.