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从 ( 1)正常对照及经庚酸睾酮治疗了 2 1周的成年白人 ,以及 ( 2 )正常对照及经GnRH拮抗剂治疗了 16～ 2 5d的成年猕猴 ,获取 2 5 μm厚甲基丙烯酸树脂包埋的睾丸组织切片。首先用无偏体视学新工具—光学体视框 ,在生精小管壁的局部垂直切片均匀抽选支持细胞核 (通过抽选其核仁 ) ,然后 ( 1)直接测量从所抽核仁中心或细胞核外侧 (基膜 )端至生精小管基膜内侧缘之间的最短直线距离 ,( 2 )并利用无偏体视学新方法—垂直转距测量法测量所抽细胞核的体积。根据是否在生精周期上半部分 (人的第Ⅰ～Ⅱ期 ,猴的第Ⅰ～Ⅵ期 )将支持细胞分成两个时相。结果显示 ,正常猴支持细胞核在上半生精周期离基膜的距离显著远于下半周期 ,这提示支持细胞核随大量生精细胞从基底向管腔的发生而移动。但在人未检出这种周期性改变 ,这可能与人生精细胞在管壁内的电螺旋状 (非节段性 )分布有关。上半和下半生精周期相比 ,人或猴支持细胞核的平均体积都无显著差异 ,但用上述治疗显著抑制精子发生后支持细胞核的平均体积都显著缩小约 2 2～ 34%。
Adult (21) adult whites were treated with (1) normal control and transheptane testosterone for 21 weeks, and (2) normal controls and adult cynomolgus monkeys treated with GnRH antagonist for 16-25 days to obtain 25 μm thick methacrylic resins Embedded testicular tissue sections. First of all, with the help of a new non-stereoscopic visualization tool, optical body frame, the supporting nuclei were drawn by local vertical section on the wall of the seminiferous tubules (by sampling their nucleoli) and then (1) Center or nucleus (basement membrane) end to the inner edge of the basement membrane between the shortest straight line distance, (2) and the use of a new method of unbiased visual method - vertical torque measurement of the volume of the cell nucleus. Support cells are divided into two phases depending on whether they are in the first half of the fertilization cycle (stage I-II of humans, stage I-VI of the monkey). The results showed that the distance between the normal monkey supporting nuclei and the basement membrane in the first half of the seminiferous cycle was significantly shorter than that in the second half, suggesting that the supporting nuclei migrate as the large number of germinal cells migrate from basal to lumen. However, this periodic change was not detected in humans, which may be related to the electric spiral (non-segmental) distribution of spermatozoa in the wall of the human body. The mean volume of supporting nuclei in either human or monkey did not differ significantly between the top half and the bottom half of the spermatogenic cell cycle. However, the average volume of supporting nuclei significantly decreased by ~2 ~ 34% after spermatogenesis with the above treatment.