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目的:研究雌激素在体外调节人脐带静脉内皮细胞(HUVEC)的生长调节致癌基因α(GROα)。方法:以体外培养的HUVEC为模型,Northern法检测CXC族趋化因于GROα mRNA;ELISA方法检测细胞表面的GROα蛋白表达;静态细胞粘附实验测定细胞表面的GROα蛋白的生理意义。结果:17β-雌二醇(0.05μmol/L)明显抑制HUVEC产生GROα mRNA和蛋白表达水平;而且17β-雌二醇抑制其蛋白表达水平显示剂量依赖性关系;雌激素受体α拮抗剂他莫昔芬(0.1μmol/L)单独使用不影响其蛋白表达,但可显著逆转17β-雌二醇抑制的GROα蛋白表达,显著逆转17β-雌二醇抑制单核细胞系细胞U937细胞粘附到HUVEC的作用达。结论:通过内皮细胞上雌激素受体α,雌激素可能功能性调节人内皮细胞的GROα的表达。
OBJECTIVE: To study the regulation of estrogen on the growth regulation of human umbilical vein endothelial cells (HUVECs) in vitro. Methods: HUVEC cultured in vitro was used as the model. The chemokine CXa chemokine was detected by Northern blot. The expression of GROα protein was detected by ELISA. The physiological significance of GROα protein was measured by static cell adhesion assay. Results: 17β-estradiol (0.05μmol / L) significantly inhibited the expression of GROα mRNA and protein in HUVECs. Moreover, the inhibitory effect of 17β-estradiol on the expression of GROα was dose-dependent. The estrogen receptor α (0.1μmol / L) alone did not affect its protein expression, but significantly reversed 17β-estradiol inhibited GROα protein expression, significantly reversed 17β-estradiol inhibited monocyte cell line U937 cells adhered to HUVEC The role of up. CONCLUSIONS: Estrogen may functionally regulate the expression of GROα in human endothelial cells via the estrogen receptor alpha on endothelial cells.