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目的 :寻求快速、简便、特异性强、敏感性高的甲型副伤寒血清学诊断方法 ;方法 :采用胶乳凝集试验 (LPS -Latex)及脂多糖 -被动血凝试验 (LPS -PHA)检测甲型副伤寒、伤寒患者血清 ,并同期检测非伤寒患者血清进行统计分析 ;结果 :单项低滴度脂多糖 -被动血凝试验及联合试验诊断甲型副伤寒符合率分别为 86.95%和 84.78% ,且分别与伤寒及非伤寒组比较有显著意义 (P <0 .0 5,P <0 .0 0 1 )。两项试验假阳性率分别为 1 2 .50 %及 0 (P <0 .0 0 1 ) ;结论 :胶乳凝集联合低滴度脂多糖 -被动血凝试验诊断甲型副伤寒简便、快速 ,有较高敏感性和特异性。
OBJECTIVE: To seek a rapid, simple, specific and sensitive method for the serological diagnosis of paratyphoid fever A. Methods: LPS-Lx and lipopolysaccharide-passive hemagglutination test (LPS-PHA) Type paratyphoid and typhoid fever, and the serum of patients with non-typhoid fever during the same period were statistically analyzed.Results: The coincidence rate of single low-titer lipopolysaccharide-passive hemagglutination test and combined test was 86.95% and 84.78%, respectively, Respectively, compared with typhoid fever and non-typhoid fever group (P <0.05, P <0.001). The false-positive rates of the two tests were respectively 12.5% and 0 (P0.01). Conclusion: The latex agglutination combined with low-titer lipopolysaccharide-passive hemagglutination test can be used to diagnose Paratyphoid A Higher sensitivity and specificity.