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青霉菌灭活菌丝体(Dry Mycelium of Penicillium chrysogenum,DMP)是工业生产青霉素的残余副产物,研究发现DMP能够提高多种作物的抗病性。本文研究了DMP对烟草BY-2悬浮细胞防卫反应的诱导作用,并初步探索其诱导机制,结果表明:DMP处理烟草BY-2悬浮细胞后,产生了活性氧迸发,在处理后30 min达到峰值;DMP诱导烟草BY-2悬浮细胞胞外基质碱性化,该变化能被蛋白激酶抑制剂K252a部分抑制;苯丙氨酸解氨酶(PAL)和过氧化物酶(POD)的活性被诱导而明显升高,分别在处理后4 h和8 h达到峰值;DMP诱导了病程相关蛋白基因PR-1a、PR-1b以及抗病信号传导途径关键基因NPR1的表达。说明DM P能够诱导烟草BY-2悬浮细胞产生抗病防卫反应,其抗病信号可能是通过水杨酸信号途径进行传导的,蛋白质磷酸化参与了该抗病信号的传导过程。
Dry Mycelium of Penicillium chrysogenum (DMP), a byproduct of industrial production of penicillin, has found that DMP can enhance the disease resistance of many crops. In this paper, we studied the induction of DMP on the defensive response of tobacco BY-2 suspension cells and explored its induction mechanism. The results showed that the reactive oxygen species (DMP) of the BY-2 suspension cells were treated with DMP, and reached the peak at 30 min after treatment ; DMP induced basophil extracellular matrix of BY-2 suspension cells, which could be partially inhibited by protein kinase inhibitor K252a; the activity of PAL and POD was induced DMP induced the expression of PR-1a, PR-1b and NPR1, a key gene of disease-resistance signal transduction pathways. The results showed that DM P could induce the resistance of BY-2 cells to disease-resistant defense reaction. The disease-resistance signal may be mediated by salicylic acid signaling pathway. Protein phosphorylation is involved in the transduction of the resistance signal.