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目的:研究神经生长因子(NGF)对大鼠前脑缺血再灌注后海马CA1区Fas蛋白表达和细胞凋亡的影响。方法:夹闭大鼠双侧颈总动脉造成前脑缺血,30 min后松夹再灌注,NGF组和生理盐水组于再灌注开始时分别肌肉注射NGF 30μg·mL~(-1)和生理盐水0.1 mL,应用免疫组化法和TUNEL法检测各组大鼠海马CA1区Fas蛋白表达和细胞凋亡。结果:再灌注后6和24 h,NGF组Fas蛋白平均吸光度值小于生理盐水组(P<0.001和P<0.05)。再灌注后48 h两组比较差异无统计学意义(P>0.5)。再灌注后6、24和48 h,NGF组TUNEL阳性细胞率均低于生理盐水组,差异有统计学意义(P<0.005)。结论:NGF可以减少大鼠脑缺血再灌注后海马CA1区Fas蛋白表达,抑制细胞凋亡,从而发挥其神经保护作用。
Objective: To investigate the effect of nerve growth factor (NGF) on the expression of Fas protein and apoptosis in the hippocampal CA1 subfield after forebrain ischemia-reperfusion in rats. Methods: The forebrain ischemia was occluded in bilateral common carotid arteries in rats. After 30 min of reperfusion, the NGF and saline groups were intramuscularly injected with NGF (30 μg · mL -1) and physiology 0.1 mL of saline. The expression of Fas protein and apoptosis in the hippocampal CA1 region were detected by immunohistochemistry and TUNEL. Results: At 6 and 24 h after reperfusion, the average absorbance of Fas protein in NGF group was lower than that in saline group (P <0.001 and P <0.05). 48 h after reperfusion, there was no significant difference between the two groups (P> 0.5). At 6, 24 and 48 h after reperfusion, the TUNEL positive rate in NGF group was significantly lower than that in saline group (P <0.005). CONCLUSION: NGF can reduce the expression of Fas protein in hippocampal CA1 area after cerebral ischemia-reperfusion in rats and inhibit the apoptosis of neurons in order to exert its neuroprotective effect.