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Background Dimethylaminoethanol has been widely used to fight against wrinkles, in the field of aesthetic medicine there is an increasing demand for safe and effective Dimethylaminoethanol-based products to counteract the ageing process. Objective To evaluate the anti-ageing effects of a new DMAE-based formulation. Methods 30 male rats were randomly allocated into treatment,D-gal ageing modeland control groups, each of which contained ten rats. Treatment group and D-gal ageing model group were subcutaneously injected with D- galactose prepared in normal saline 125mg·kg-1·d-1 for 42d. Control groups were injected with normal saline for 42 d with same method and dose. From the 18th day,after shaving their hair,the treatment grouprats were injected thisnew DMAE-based formulation at a dose of 1ml per week for 4 weeks in the Dermis of two sides hip skin mark zone.Meanwhile,D-gal ageing model group rats were administrated the same volume of normal saline with same method. Skin specimens were obtained 3days after the last treatment. Dermal collagen density and dermal thickness were evaluated by H&E and Masson-trichrome staining. And mRNA expressions of TGFβ1, Smad3, Type I,Type III Pro-collagen,TIMP-1, MMP-1,were assessed by Real-time quantitative polymerase chain reaction. Results Dermal thickness, dermal collagen density and hydroxyproline content in treatment group increased significantly comparing with D-gal ageing model group. No differences were found in mRNA expression of MMP-1 and Type III Pro-collagen between the treatment group and D-gal ageing model group. In addition, mRNA expression of TGFβ1, Type I Pre-collagen , TIMP1 and smad3 in treatment group were significantly up-regulated in contrast with D-gal ageing model and control group. Conclusion This new DMAE-based formulationcould generate anti-ageing effects by activating collagen synthesisthrough TGF-β1/Smads signaling pathway.