论文部分内容阅读
目的:肿瘤细胞对放疗的抗辐射性常与凋亡通路障碍有关。因此,探讨了Fas信号转导通路是否在高度耐受辐射的人胶质细胞瘤中诱导细胞凋亡。方法:1将携带有荧光素酶基因和突变型p53cDNA(在175bp位点)的逆转录病毒载体转染至无p53基因突变的胶质瘤细胞株U-87MG中。培养细胞经剂量率
OBJECTIVE: The radioresistance of tumor cells to radiotherapy is often related to the apoptosis pathway. Thus, we investigated whether the Fas signaling pathway induces apoptosis in human glioma cells that are highly resistant to radiation. Methods: 1 The retroviral vector carrying luciferase gene and mutant p53 cDNA (at 175bp site) was transfected into glioma cell line U-87MG without p53 gene mutation. Culturing cells via dose rate