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滤纸法(FPT)测试单个棉蚜酯酶活性可以检测棉蚜对有机磷杀虫剂的抗性。为准确测试不同杀虫剂的抗性水平,本文改进了FPT的方法,测定了甲基对硫磷、久效磷和氧乐果对棉蚜个体α-NA酯酶的抑制作用,比较了BCA(敏感)和GCA(抗性)种群的抑制频率。结果指出二种不同浓度的杀虫剂对BCA种群的抑制频率明显高于GCA种群。特别在测定久效磷对经久效磷处理后棉蚜F_2代个体的抑制率,仅有一个棉蚜F_1代的抑制率达19％,其他均无抑制作用,而对未经处理的棉蚜F_1代个体的抑制率均可达70％-90％,但在α-NA酯酶活性上差异不明显。测定同地区三种有机磷杀虫剂对已喷过杀虫剂的棉蚜种群,其个体酯酶抑制频率明显低于未喷杀虫剂的棉蚜种群。这些均表明用FPT测定有机磷杀虫剂对棉蚜个体α-NA酯酶的抑制频率可作为抗性测报技术。
A single cotton aphid esterase activity assay by the filter paper method (FPT) detects the resistance of cotton aphids to organophosphate insecticides. In order to accurately test the resistance of different insecticides, this paper improves the method of FPT, and measures the inhibitory effect of methyl parathion, monocrotophos and omethoate on the α-NA esterase of cotton aphid individuals. The BCA (Sensitive) and GCA (resistant) populations. The results showed that the two different concentrations of pesticides on the BCA population suppression frequency was significantly higher than the GCA population. In particular, the inhibitory rate of monocrotophos to F 2 generation of cotton aphid after treatment with monocrotophos was only 19%, and no inhibition was found in other F 1 generation of cotton aphid. On behalf of the individual inhibition rate of up to 70% -90%, but α-NA esterase activity was not significantly different. Determination of the same area three organophosphate insecticides have been sprayed insecticides cotton aphid population, the individual esterase inhibition frequency was significantly lower than the insecticide-free cotton aphid population. All of these indicate that using FPT to determine the inhibitory rate of organophosphate insecticides on α-NA esterase of cotton aphid individuals can be used as a measure of resistance.