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一、材料和方法１．材料 ＳＴＫ质粒由ＭｏｏｌｔｅｎＦＬ教授惠赠。大鼠神经胶质瘤细胞Ｃ６由上海长征医院王驹博士，ＰＡ３１７细胞由上海肿瘤研究所提供，Ｈｅｌａ细胞由武汉生物制品研究所惠赠，大肠杆菌ＤＨ５α为本实验室保存。质粒纯化试剂盒、ＤＭＥＭ培养基、脂质体
First, materials and methods Materials STK plasmid was a gift from Professor MooltenFL. The rat glioma cell line C6 was provided by Shanghai Long March Hospital Dr. Wang Wei, PA317 cell by the Shanghai Cancer Institute, Hela cells by the Wuhan Institute of Biological Products, and E. coli DH5α by the laboratory. Plasmid Purification Kit, DMEM Medium, Liposomes