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本研究鉴定到一份水稻苗期白化转绿突变体,该突变体在幼苗的2叶到4叶期会出现叶片边缘白化,4叶期后转绿,对突变体和野生型农艺性状比较研究表明,前期白化不会对植株生长发育及产量等相关性状产生影响;同时,对两者叶绿体超微结构对比分析发现,突变体叶绿体发育在白化期受到一定程度的抑制。通过构建F2分离群体,调查了部分F2单株表型,结果表明白化型和野生型单株符合1:3的孟德尔单基因分离比,遂将该基因命名为Gra3;利用分离群体分析法(bulked segregant analysis,BSA)和隐性群体分析法(recessive-class analysis,RCA)最终将Gra3定位于RM14436和RM14450之间,对应日本晴基因组片段125 kb。该性状可作为一个形态学标记应用于水稻生产。
In this study, we identified a white-greening and greening mutant in rice seedling stage. The mutant showed leaf edge whitening at the second leaf to the fourth leaf stage and greening after the fourth leaf stage, and compared the mutants and wild-type agronomic traits The results showed that pre-albino had no effect on plant growth, yield and other related traits. Meanwhile, comparative analysis of chloroplast ultrastructure between the two showed that the chloroplast development of the mutant was inhibited to a certain extent during the albino stage. By constructing F2 segregating population, we investigated the phenotypic characteristics of some F2 plants. The results showed that the male-sterile and wild-type plants were aligned with Mendelian segregation ratio of 1: 3, named Gra3. Finally, Gra3 was located between RM14436 and RM14450, corresponding to 125 kb of Nipponbare genomic fragment, using bulked segregant analysis (BSA) and recessive-class analysis (RCA). This trait can be used as a morphological marker in rice production.