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目的β防御素3(HBD3)除了具有天然免疫功能,还发挥调节后天适应性免疫的作用。本研究拟通过支气管哮喘患儿、急性上呼吸道感染患儿及正常儿血浆HBD3浓度的比较,探讨HBD3在哮喘发病中的作用。方法研究对象选择2009年4月到12月在哈尔滨医科大学附属第一医院儿科就诊病例共81例,其中哮喘组21例,为急性发作期支气管哮喘患儿,感染组29例,为急性上呼吸道感染患儿,正常对照组31例,为健康体检儿。采用酶联免疫吸附试验(ELISA)法进行血浆HBD3水平测定。同时测定血嗜酸性粒细胞总数和白细胞数,9例哮喘儿测定了血清IgE浓度。结果血浆HBD3浓度正常儿为(8.028±1.078)μg/ml,哮喘患儿(12.212±1.124)μg/ml感染患儿(8.976±1.110)μg/ml,哮喘患儿与急性呼吸道感染及正常儿3组总体比较,血浆HBD3浓度明显升高,差异有显著性(F=4.448,P<0.05);哮喘组与正常组比较,差异显著,P<0.01;哮喘组与感染组比较,差异有显著性P<0.05;感染组与正常组比较,差异无显著性P>0.05;同时发现血浆HBD3水平与血清总IgE,血嗜酸性粒细胞及白细胞水平无显著相关性。结论支气管哮喘发作时HBD3表达升高,HBD3可能是超越感染之外,参与哮喘发病的独立因素。HBD3是否始动因素尚待进一步探索。
Purpose β-defensin 3 (HBD3), in addition to its innate immune function, also plays a role in the regulation of adaptive immunity. This study intended to compare the plasma concentrations of HBD3 in children with acute asthmatic bronchial asthma, children with acute upper respiratory tract infection and normal subjects, and to explore the role of HBD3 in the pathogenesis of asthma. Methods Subjects selected 81 cases of pediatric visits in the First Affiliated Hospital of Harbin Medical University from April 2009 to December 2009, of which 21 cases were asthma group, 29 cases were acute bronchial asthma and 29 cases were infected with acute upper respiratory tract Infected children, the normal control group of 31 cases, for the health examination of children. Plasma HBD3 levels were measured by enzyme-linked immunosorbent assay (ELISA). At the same time, the total number of blood eosinophils and the number of white blood cells were measured. Serum IgE concentrations were measured in 9 asthmatic children. Results The plasma levels of HBD3 were (8.028 ± 1.078) μg / ml and (12.212 ± 1.124) μg / ml in children with asthma (8.976 ± 1.110 μg / ml, Compared with the normal group, the difference was statistically significant (P <0.01). There was significant difference between the asthma group and the infection group (P <0.05) P <0.05. There was no significant difference between the infected group and the normal group (P> 0.05). Meanwhile, there was no significant correlation between the plasma level of HBD3 and serum total IgE, blood eosinophils and leukocytes. Conclusions The expression of HBD3 is elevated in the onset of bronchial asthma. HBD3 may be an independent factor that is beyond the infection and participates in the pathogenesis of asthma. Whether HBD3 is the starting factor remains to be further explored.