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由于β-外毒素对家蝇三龄幼虫的毒性和几种抗癌药相似,且能抑制昆虫脱皮或变态时RNA和DNA的合成,为此,我们进行了体内抗肿瘤实验。 (一)β-外毒素对小鼠S18腹水型癌细胞数的影响实费用CFW小鼠,体重18～22克,雌雄皆有,在无菌条件下给每只动物腹腔接种生长7天的小鼠S180腹水型瘤细胞5×10~6个,接种后24小时随机分组给药,对照组小鼠腹腔注射生理盐水O.2毫升/只,实验组小鼠腹腔注射不同剂量的β-外毒素,每日一次,连续给药5天,末次给药的次日处死动物,取出全部腹水,用生理盐水反复冲洗腹腔5次,再用血细胞计算板计数全腹腔癌细胞总数,观察结果见表
Because β-exotoxins are similar to several third-instar larvae of Musca domestica and are similar to several anti-cancer drugs and can inhibit the synthesis of RNA and DNA during exfoliation or metamorphosis of insects, we conducted in vivo antitumor experiments. (I) Influence of β-exotoxin on the number of ascites-type cancer cells in mice S18 Cost CFW mice, weighing 18-22 grams, both male and female, were inoculated intraperitoneally with each animal under aseptic conditions for 7 days. Rat S180 ascites tumor cells were 5×10-6 cells. Randomly divided into groups 24 hours after inoculation. Control mice were injected intraperitoneally with 0.2 ml of normal saline. The mice in experimental group were given different doses of β-exotoxin by intraperitoneal injection. , Once daily, continuous administration for 5 days, the animals were sacrificed on the following day of the last administration, all ascites were taken out, and the abdominal cavity was repeatedly washed with saline 5 times, and the total number of cancer cells in the abdominal cavity was counted using a blood cell counting board.