论文部分内容阅读
目的研究红松松针中的黄酮类成分,为松属植物的化学分类学研究提供依据。方法采用反复硅胶、聚酰胺、ODS、Sephadex LH-20柱色谱等方法进行分离纯化,根据理化性质和1H-NMR、13C-NMR等技术对分离得到的化合物进行结构鉴定。结果从红松松针中分离得到7个化合物,分别鉴定为蛇葡萄素4′-O-β-D-吡喃葡萄糖苷[ampelopsin 4′-O-β-D-glucopyranoside,1]、槲皮素3-O-α-L-呋喃阿拉伯糖苷[quercetin3-O-α-L-arabinofuranoside,2]、山柰酚3-O-α-L-呋喃阿拉伯糖苷[kaempferol3-O-α-L-arabinofuranoside,3]、山柰酚3-O-β-D-吡喃葡萄糖苷[kaempferol3-O-β-D-glu-copyranoside,4]、5,7,8,4′-四羟基-3-甲氧基-6-甲基黄酮8-O-β-D-吡喃葡萄糖苷[5,7,8,4′-tetra-hydroxy-3-methoxy-6-methylfavone8-O-β-D-glucopyranoside,5]、山柰酚3-O-(5″-O-反式-阿魏酰基)-α-L-呋喃阿拉伯糖苷[kaempferol3-O-(5″-O-E-feruloyl)-α-L-arabinofuranoside,6]、山柰酚3-O-(5″-O-反式-对-香豆酰基)-α-L-呋喃阿拉伯糖苷[kaempferol3-O-(5″-O-E-p-coumaroyl)-α-L-arabi-nofuranoside,7]。结论化合物1-3为首次从松属植物中分离得到,4-7为首次从该种植物中分离得到。
Objective To study the flavonoids in Pinus koraiensis pine needles and provide the basis for the chemical taxonomy of Pinus. Methods The compounds were separated and purified by repeated silica gel, polyamide, ODS and Sephadex LH-20 column chromatography. The structures of the isolated compounds were identified by 1H-NMR and 13C-NMR. Results Seven compounds were isolated from pine needles and identified as ampelopsin 4’-O-β-D-glucopyranoside (1), quercetin 3-O-α-L-arabinofuranoside (2), kaempferol 3-O-α-L-arabinofuranoside 3], kaempferol 3-O-β-D-glucopyranoside (4), 5,7,8,4’-tetrahydroxy-3-methoxy 6-methylflavone 8-O-β-D-glucopyranoside [5,7,8,4’-tetra-hydroxy-3-methoxy-6-methylfavone8-O- ], Kaempferol 3-O- (5 “-OE-feruloyl) -alpha-L-arabinofuranoside, 6], kaempferol 3-O- (5 ”-OEp-coumaroyl) -alpha-L -rabi-nofuranoside, 7]. Conclusion Compounds 1-3 were isolated from Pinus for the first time and 4-7 were isolated from this species for the first time.