论文部分内容阅读
因子依赖性的人T 细胞系已有十多年的建株历史,然而,建立因子依赖性的人B 细胞系至今未获成功。通过采用抗CD_(40)单克隆抗体和IL-4共同作用,建立了可以在体外长期培养的因子依赖性的B 细胞系。CD_(40)是一种表达于B 淋巴细胞、上皮细胞和一些肿瘤细胞系、分子量为45KD～50KD 膜转移糖蛋白。从外周血、骨髓、脾和扁桃体中分离的休止型B 细胞系共同孵育,当加入抗CD_(40)单克隆抗体和IL-4时,正常人休止型B 淋巴细胞可以进入持续增殖阶段。由于抗CD_(40)的促B 细胞增殖效应依赖于Ltk~-细胞系膜上FcγRⅡ/CDW32的存在,以及可溶性抗CD_(40)单克隆抗体,也不具备刺
Factor-dependent human T cell lines have a history of more than ten years of establishment, however, the establishment of factor-dependent human B cell lines has so far not been successful. By using anti-CD40 mAb in combination with IL-4, a factor-dependent B cell line that can be cultured in vitro for a long time was established. CD40 (40) is a membrane-derived glycoprotein with a molecular mass of 45KD ~ 50KD expressed in B lymphocytes, epithelial cells and some tumor cell lines. Incubate B cell lines isolated from peripheral blood, bone marrow, spleen and amygdala are co-incubated. Normal human resting B lymphocytes can enter the proliferative phase when anti-CD40 monoclonal antibody and IL-4 are added. Because the anti-CD40 (40) pro-B cell proliferation effect depends on the presence of FcγRII / CDW32 on Ltk ~ - cell lineage and the soluble anti-CD40 monoclonal antibody,