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作者试图通过确定蜡样芽胞杆菌肠毒素实验性培养合成的最适条件,以阐明食品中肠毒素产生的条件。把蜡样芽胞杆菌纯培养液以不同的接种量(1%、5%和10%)接种于不同葡萄糖含量(0、1%、5%和25%)和不同pH(5.0,5.5、6.0、6.5、7.0、7.5、8.0和8.5)的CA液体培养基中,在不同温度(20、25、32、37和42℃)下,用不同的振速(200、400、600和800转/分)和通入不同流速(0、20、50、100、300和944 ml/分)的空气,培养4～9小时后,分别以5ml培养液置于离心机中,高速离心10分钟,
The authors attempted to elucidate the conditions for the production of enterotoxins in foods by determining the optimum conditions for the experimental culture of B. cereus enterotoxins. Pure cultures of B. cereus were inoculated with different inoculum sizes (0, 1%, 5% and 25%) and different pH (5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0 and 8.5) at different temperatures (20, 25, 32, 37 and 42 ° C) with different vibrations (200, 400, 600 and 800 rpm ) And air at different flow rates (0, 20, 50, 100, 300 and 944 ml / min) were introduced. After 4 to 9 hours of culture, the cells were respectively placed in a centrifuge with 5 ml of culture medium, centrifuged at high speed for 10 minutes,