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目的:观察清热解毒中药-四藤方对肝癌细胞Bel-7402增殖和凋亡的影响。方法:不同浓度四藤方处理人肝癌细胞Bel-7402、人肝细胞HL-7702,WST-8法检测细胞增殖,流式细胞术检测细胞凋亡,Hoechst 33258染色检测细胞凋亡形态变化,Western Blot法检测半胱天冬酶-3(caspase-3)活化。结果:与对照组比较,四藤方对人肝细胞HL-7702增殖无明显影响;100～400μg.mL-1四藤方作用24 h可显著抑制肝癌Bel-7402细胞增殖,促使细胞Bel-7402凋亡,呈现细胞凋亡形态,并可活化Bel-7402细胞caspase-3。结论:四藤方可以抑制Bel-7402细胞增殖,促Bel-7402细胞凋亡,并可能与活化caspase-3相关。
Objective: To observe the effect of Qingre Jiedu Chinese medicine - Si Teng Fang on the proliferation and apoptosis of hepatocellular carcinoma Bel-7402 cells. METHODS: Bel-7402 human hepatocellular carcinoma cells were treated with different concentrations of Si Teng Fang, the proliferation of HL-7702 cells was detected by WST-8 and the apoptosis of cells was detected by flow cytometry. The morphological changes of apoptotic cells were detected by Hoechst 33258 staining. Blot assay for caspase-3 activation. Results: Compared with the control group, SiTuFang had no significant effect on the proliferation of human hepatocellular HL-7702 cells. After treated with 100 ~ 400μg.mL-1 SiTuFang for 24 hours, the proliferation of Bel-7402 hepatoma cells was significantly inhibited, and Bel-7402 Apoptosis, morphological changes of apoptotic cells and activation of caspase-3 in Bel-7402 cells. Conclusion: Si Teng Fang can inhibit the proliferation of Bel-7402 cells and induce the apoptosis of Bel-7402 cells, which may be related to the activation of caspase-3.