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背景本研究旨在评估第一次供血者中丙型肝炎病毒(HCV)抗体和RNA流行状况以及HCV~-基因型分布。 研究设计和方法使用第三代ELISA试剂筛选34725名供血者的抗-HCV,重复反应者用第二、三代重组蛋白免疫印迹试验和PCR检测。PCR阳性者进行基因分型。全部样品均进行丙氨酸转氨酶筛选。 结果ELISA检测阳性率为0.55％。PCR检测表明在ELISA检测阳性的样品中69/183(38％)含有
Background The purpose of this study was to evaluate the prevalence of hepatitis C virus (HCV) antibodies and RNA and the HCV genotype distribution in first-time donors. Study Design and Methods Screening 3725 donors for anti-HCV using a third-generation ELISA kit, replicate responders with second and third-generation recombinant protein immunoblot assays and PCR assays. PCR positive for genotyping. All samples were alanine aminotransferase screening. Results The positive rate of ELISA test was 0.55%. PCR assays showed that 69/183 (38%) was contained in the ELISA-positive samples