论文部分内容阅读
用十二指肠钩虫幼虫人工感染幼犬后14d,从犬肠道获得体长约2mm、生殖器官尚未发育的十二指肠钩虫童虫共106条,经犬血清和等量的犬血清RPMI 1640培养基培养后,童虫生长发育良好,存活率达90％,生殖器官逐渐显现并发育成熟,雌虫在培养后24d开始排卵,最高日产卵数7000个。50％的童虫存活54d以上,最长可存活154d,体长达8～9mm。有两对童虫在体外发育成熟后出现交配,雌虫排出受精虫卵,将该虫卵培养后,并获得感染期钩虫幼虫,此种幼虫再感染幼犬,首次检获十二指肠钩虫成虫436条。
At 14 days after infection with pupae of larva of Duodenal hookworm, a total of 106 duodenal hookworm sires were obtained from the canine intestine with a length of about 2 mm and reproductive organs were not well developed. The canine serum and the same amount of canine serum RPMI After 1640 medium was cultured, the growth and development of Schistosoma japonicum was good with the survival rate of 90%. The reproductive organs gradually developed and matured. The female started ovulation 24 days after the culture and the highest number of eggs per day was 7000. 50% of the worms survived more than 54d, the longest survival 154d, body length of 8 ~ 9mm. There are two pairs of female pests in vitro maturity after mating, females excreted fertilized eggs, the eggs were cultured, and get infected hookworm larvae, such larvae and puppies re-infected, the first seizure of adult duodenal hookworm 436 articles.