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轮状病毒是婴儿和小儿急性胃肠炎的主要病原体,在温带地区的较冷季节里,它占50％。1976年12月～1978年1月,墨西哥城的两所医院242名急性胃肠炎患儿的粪便中,61名(25％)由电镜检出轮状病毒。墨西哥城地处温带,轮状病毒感染发病高峰在10月,秋季高于冬季。过去加拿大的报告说明,多伦多的轮状病毒感染,冬季发病最高。将患儿粪便提取物在45％蔗糖溶液中做不连续超速离心,每分钟20万转,离心1小
Rotavirus is the major causative agent of acute gastroenteritis in infants and children, accounting for 50% of the colder months in temperate regions. From December 1976 to January 1978, out of 242 children with acute gastroenteritis in two hospitals in Mexico City, rotavirus was detected by electron microscopy in 61 (25%) of the stools. Mexico City is located in temperate, rotavirus infection peak incidence in October, autumn is higher than in winter. The past Canadian report shows that rotavirus infection in Toronto has the highest incidence in winter. Children stool extract in 45% sucrose solution discontinuous ultracentrifugation, 200,000 revolutions per minute, centrifuge 1 small