论文部分内容阅读
目的观察新生儿缺氧缺血性脑病(HIE)患儿外周血T细胞亚群和膜白介素-2受体(mIL-2R)表达,探讨其临床意义。方法安徽省淮南市妇幼保健院新生儿科2002年5月至2003年10月收治HIE患儿32例。以Ficoll-Hypaque常规分离外周血单个核细胞(PBMC),用生物素-链霉亲和素(BSA)系统检测新生儿HIE及足月正常新生儿生后第1、3、7日CD3+、CD4+、CD8+、CD4+/CD8+阳性率,及PHA诱导前后mIL-2R表达水平。结果新生儿HIE患儿生后第1日CD3+、CD4+、CD8+阳性率及CD4+/CD8+、静息期和诱导期mIL-2R表达水平与正常对照组相比,差异有显著性(P<0.01或P<0.05);生后第3日与正常对照组相比,差异有显著性(P<0.05);生后第7日CD3+、CD4+、CD8+与正常对照组相比,差异有显著性(P<0.05),CD4+/CD8+与正常对照组相比,差异无显著性(P>0.05)。在不同程度HIE患儿中,以重度HIE患儿细胞免疫功能异常最明显。结论新生儿免疫细胞幼稚、未分化成熟、表达水平偏低,HIE病理过程有细胞免疫的改变及参与,HIE患儿存在一定程度的细胞免疫功能紊乱。
Objective To observe the expression of T cell subsets and membrane interleukin-2 receptor (rIL-2R) in peripheral blood of neonates with hypoxic-ischemic encephalopathy (HIE) and to investigate its clinical significance. Methods 32 cases of HIE children were admitted to Department of Neonatology, Huainan MCH Hospital from May 2002 to October 2003. Peripheral blood mononuclear cells (PBMCs) were routinely isolated from Ficoll-Hypaque cells, and the levels of CD3 +, CD4 + and CD8 + were detected in neonates with HIE and full-term normal newborns on the 1st, 3rd, and 7th day after birth by using biotin-streptavidin (BSA) , The positive rate of CD8 +, CD4 + / CD8 +, and the expression of mIL-2R before and after PHA induction. Results The positive rate of CD3 +, CD4 +, CD8 + and the expression of mIL-2R at resting stage and induction stage in newborn infants with HIE on the 1st day after birth were significantly higher than those in the normal control group (P <0.01 or (P <0.05). There was a significant difference between the 3rd day after birth and the normal control group (P <0.05). The difference of CD3 +, CD4 +, CD8 + between the 7th day and the normal control group was significant <0.05). There was no significant difference between CD4 + / CD8 + and normal control group (P> 0.05). In different degrees of HIE children, severe HIE children with abnormal cellular immune function most obvious. Conclusion The immune cells of infants are immature, undifferentiated and mature, and their expression levels are low. The cellular immunity changes and participate in the pathological process of HIE. There is a certain degree of cellular immune dysfunction in HIE infants.